| Reactivity | HuSpecies Glossary |
| Applications | Binding Activity |
| Format | Carrier-Free |
| Details of Functionality | Measured by its ability to bind human C1q in a functional ELISA. |
| Source | E. coli-derived human HABP1/C1QBP protein His75-Gln282, with an N-term 2-Met tag and a C-terminal 6-His tag |
| Accession # | |
| N-terminal Sequence | Met |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | C1QBP |
| Purity | >85%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
|
| Theoretical MW | 24.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 34 kDa, reducing conditions |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. |
| Purity | >85%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile deionized water. |
Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is an ubiquitous acidic glycoprotein that functions both in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 73 amino acid (aa) N-terminal propeptide (3). The 32 kDa mature human HABP1, which contains a MAM33-like sequence, shares 90% aa sequence identity with mouse and rat HABP1. HABP1 assembles into a doughnut shaped trimer with the negatively charged residues asymmetrically distributed on one face and lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 3, 6 - 8). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (9 - 14). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (15).
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