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Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF

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2 μg/lane of Recombinant Human Guanylyl Cyclase C Fc Chimera Protein (Catalog # 10575-GC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Human Guanylyl Cyclase C/GUCY2C
(Ser21-Gln430)
Accession # P25092.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Ser21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-115 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF

  • DIAR6
  • EC 4.6.1
  • GCC
  • GC-C
  • guanylate cyclase 2C (heat stable enterotoxin receptor)
  • Guanylate Cyclase C
  • Guanylyl Cyclase C
  • GUC2C
  • GUC2CEC 4.6.1.2
  • GUCY2C
  • heat-stable enterotoxin receptor
  • hSTAR
  • Intestinal guanylate cyclase
  • MUCIL
  • STA Receptor
  • StAR
  • STARSTA receptor

Background

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family expressed by intestinal epithelial cells from the duodenum to rectum. It was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (1). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (2-4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a kinase and GC catalytic domain. The ECD of human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. Endogenous ligands of GUCY2C include guanylin and uroguanylin (5). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis. GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (5-7).
  1. Lucas K. et al. (2000) Pharmacol. Rev. 52:375.
  2. Potter, L.R. (2011) Cell Signal. 23:1921.
  3. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  4. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  5. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  6. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  7. Gong, R. et al. (2011) Science 333:1642.

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