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Recombinant Human GDF-1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
6937-GD-010/CF
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Recombinant Human GDF-1 Protein Summary

Details of Functionality
Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 50 ng/mL of Recombinant Human GDF‑1 can effectively induce Smad2 phosphorylation.
Source
Chinese Hamster Ovary cell line, CHO-derived human GDF-1 protein
Asp254-Arg372
Accession #
N-terminal Sequence
Asp254
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GDF1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
6937-GD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GDF-1 Protein

  • DORV
  • DTGA3
  • embryonic growth/differentiation factor 1
  • GDF1
  • GDF-1
  • growth differentiation factor 1

Background

Growth and Differentiation Factor 1 (GDF‑1) is a 12 ‑ 15 kDa secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that contains a 29 amino acid (aa) signal peptide, a 224 aa propeptide, and a 119 aa mature peptide which is released following proteolysis at a dibasic cleavage site (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7 ‑ 9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).
  1. Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
  2. Wall, N.A. et al. (2000) Dev. Biol. 227:495.
  3. Andersson, O. et al. (2007) Dev. Biol. 311:500.
  4. Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
  5. Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
  6. Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
  7. Andersson, O. et al. (2006) Dev. Biol. 293:370.
  8. Tanaka, C. et al. (2007) Genes Dev. 21:3272.
  9. Cheng, S.K. et al. (2003) Genes Dev. 17:31.
  10. Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.

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Publications for GDF-1 (6937-GD)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol GDF1
Uniprot