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Recombinant Human EGFR Isoform vIII Protein, CF

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Summary
Reactivity HuSpecies Glossary
Format
Carrier-Free

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Recombinant Human EGFR Isoform vIII Protein, CF Summary

Details of Functionality
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human EGFR protein
Leu25-Ser378 (Asn273Lys), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu25
Protein/Peptide Type
Innovator Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity not tested
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
65 - 80 kDa, reducing conditions
Publications
Read Publications using
9565-ER in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGFR Isoform vIII Protein, CF

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain (1-3). Human EGFR shares 90% identity with mouse and rat EGFR. The amino acid 25-378 of EGFR encodes the most common extracellular domain truncation mutant, termed EGFRvIII. It has been proved that EGFRvIII is relevant in a number of cancer types. EGFRvIII is tumor specific, and has not been found in normal tissues. EGFRvIII is highly tumorigenic, and can cause aggressive tumor phenotype, with increased invasion, proliferation, angiogenesis, and evasion of apoptosis. This EGFRvIII-mediated enhanced tumourigenicity combined with the lack of EGFRvIII expression in normal tissue makes it an ideal therapeutic targets (4).
  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
  4. Kuan, C. T. et al. (2001) Endocrine-related cancer 8:83.

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Recombinant Human
EGFR Isoform vIII Pro ...
9565-ER
Recombinant Human EGFR Isoform vIII Protein, CF
Species: Hu

Publications for EGFR (9565-ER)(2)

We have publications tested in 2 confirmed species: Mouse, N/A.

We have publications tested in 2 applications: ELISA Capture, Surface Plasmon Resonance.


Filter By Application
ELISA Capture
(1)
Surface Plasmon Resonance
(1)
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Filter By Species
Mouse
(1)
N/A
(1)
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