Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein Summary
Details of Functionality |
Measured
by flow cytometry for its ability to bind Human DLL3 Antibody conjugated
fluorescent beads at 5.00-20.0 µg/mL (100 µL/well). Please Note:
Optimal dilutions should be determined by each laboratory for each application. |
Source |
Human embryonic kidney cell, HEK293-derived human DLL3 protein Human DLL3 (Ala27-Ala479) Accession # Q9NYJ7.1 | HP | GGGSGGGSGGGS | 6-His tag | N-terminus | | | C-terminus | |
|
Accession # |
|
N-terminal Sequence |
Ala27 |
Structure / Form |
Labeled with Alexa Fluor® 647 Excitation
Wavelength: 650 nm Emission Wavelength: 668 nm |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<1.0 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
52-62 kDa, under reducing conditions. |
Packaging, Storage & Formulations
Storage |
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 6 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 3 months, -20 to -70 °C under sterile conditions after opening.
|
Buffer |
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein
Background
Delta-like
protein 3 (DLL3) is a transmembrane protein that belongs to the
Delta/Serrate/Lag-2 (DSL) family of Notch ligands (1). Mature human DLL3
consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL
domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain (2). Within the ECD, human DLL3 shares 86% aa
sequence identity with mouse and rat DLL3. DLL3
is known as a divergent DSL ligand since it does not function in similar manner
as the rest of DSL ligands. It does not activate Notch signaling through
trans-activation, but autonomously attenuates signaling induced by other DSL ligands
(3). A loss-of-function mutation of
DLL3 is linked to axial skeletal defects in the spondylocostal dysplasia,
which is linked to abnormal Notch signaling (4). DL‑3 promotes proliferation and inhibits
apoptosis of cancer cells. The proliferative effect mediated by DLL3 is
thought to be due to increased Akt phosphorylation in cancer cells (5).
-
Dunwoodie, S. L. et al. (1997) Development 124:3065.
- Bulman, M. P. et al. (2000) Nat. Genet. 24:438.
- Ladi, E. et al. (2005) J. Cell Biol. 170:983.
- Dunwoodie, S.L. et al. (2002) Development 129:1795.
- Deng S.M. et al. (2017) Biochem. Biophys. Res. Commun. 483:488.
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