Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells.<br />The ED<sub>50</sub> for this effect is typically 0.6-3.6 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.<br /><br /> |
Accession # | |
N-terminal Sequence | Leu23 |
Protein/Peptide Type | Recombinant Proteins |
Gene | IL2RG |
Endotoxin Note | <0.100 EU per 1 µg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 28.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 40 kDa, reducing conditions |
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Publications |
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Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution Instructions | Reconstitute at 500 μg/mL in sterile PBS. |
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.
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