| Reactivity | HuSpecies Glossary |
| Applications | Enzyme Activity |
| Format | Carrier-Free |
| Details of Functionality | Measured by the ability to catalyze the oxidation of 4-nitrobenzaldehyde. The specific activity is >6000 pmol/min/μg, as measured under the described conditions. |
| Source | E. coli-derived human Aldehyde Dehydrogenase 3-A1/ALDH3A1 protein Ser2-His453, with an N-terminal Met and 6-His tag |
| Accession # | |
| N-terminal Sequence | Met |
| Protein/Peptide Type | Recombinant Enzymes |
| Gene | ALDH3A1 |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 51-56 kDa, reducing conditions |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
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| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
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| Assay Procedure |
*Adjusted for Substrate Blank **Using the extinction coefficient 6270 M-1cm-1 ***Using the path correction 0.32 cm Note: the output of many spectrophotometers is in mOD Per Well:
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Aldehyde Dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that catalyze the oxidation of endogeneously produced and exogeneous aldehydes to their corresponding acids (1). They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and in lipid peroxidation. ALDH3A1 is also known as stomach aldehyde dehydrogenase. It exists as a homodimer, and prefers NADP+ over NAD+ as its co‑factor (2). It preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates (3). The enzyme is highly expressed in stomach and cornea (2, 4). In the cornea, its proposed roles have been to absorb UV‑light, reduce oxidative damage, maintain corneal refractive and transparence properties, and display chaperone-like activity (4, 5). It also has been identified as a lung cancer biomarker (6).
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