![Western Blot: RBM3 Antibody [NBP1-36980] - Analysis of RBM3 in HepG2 lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RBM3-Antibody-Western-Blot-NBP1-36980-img0001.jpg "Western Blot: RBM3 Antibody [NBP1-36980] - Analysis of RBM3 in HepG2 lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DRYSGGNYRDNYDN corresponding to C-Terminus according to NP_006734.1. |
| Epitope | C Terminus |
| Predicted Species | Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RBM3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 17 kDa band observed in HepG2 lysates (calculated MW of 17.2 kDa band according to NP_006734.1).
An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-36980 | Applications | Species |
|---|---|---|
| Sureban SM, Ramalingam S, Natarajan G et al. Translation regulatory factor RBM3 is a proto-oncogene that prevents mitoticcatastrophe. Oncogene 2008-07-31 [PMID: 18427544] |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | RBM3 |
| Entrez | |
| Uniprot |
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