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Rab5a Antibody

Images

 
Western Blot: Rab5a Antibody [NBP1-58880] - Human Muscle.
Immunohistochemistry: Rab5a Antibody [NBP1-58880] - Zebra gut epithelial cells. Green: Primary antibody (1:5000). Red: actin (1:300). Blue: DAPI. Image submitted by Michel Bagnat, Duke University Medical Center. See ...read more
Immunohistochemistry: Rab5a Antibody [NBP1-58880] - Zebra gut epithelial cells. Green: Primary antibody (1:5000). Red: actin (1:300). Blue: DAPI. Image submitted by Michel Bagnat, Duke University Medical Center. See ...read more

Product Details

Summary
Reactivity Hu, Mu, Ma, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Rab5a Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to RAB5A(RAB5A, member RAS oncogene family). The peptide sequence was selected from the middle region of RAB5A. Peptide sequence KTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCS. The peptide sequence for this immunogen was taken from within the described region.
Marker
Early Endosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB5A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1.0 ug/ml
Reviewed Applications
Read 1 Review rated 4
using
NBP1-58880 in the following applications:

Publications
Read Publication using
NBP1-58880 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29018038).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Rab5a Antibody

  • RAB5A, member RAS oncogene family
  • RAB5RAS-associated protein RAB5A
  • ras-related protein Rab-5A

Background

RAB5A is required for the fusion of plasma membranes and early endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab5a Antibody (NBP1-58880)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Rab5a Antibody (NBP1-58880) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-58880:
Filter by Applications
Immunofluorescence (IF)
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Other Rab5a NBP1-58880
Enlarge
4
reviewed by:
NIKHIL VAD
Immunofluorescence (IF) Human 07/17/2018
View

Summary

ApplicationOther
Sample TestedA172 Glioblastoma cells
SpeciesHuman
LotQC27615-100415

Comments

CommentsGBM cells grown on uncoated coverslips were fixed with 4% paraformaldehyde (ice cold) for 15 min. Following fixation, cells were rinsed thrice with PBS, permeabilized with ice-cold methanol for 10 min at 4C. Following permeabilization, coverslips were rinsed thrice with PBS, incubated with blocking buffer (5% goat serum, 0.3% Triton-X 100) for 2 h at RT. Samples were then incubated with Rab5 antibody (1:200) at 4C overnight. Following morning, coverslips were washed thrice with PBS, incubated with anti-rabbit Alexa-Fluor 488 secondary antibody (1:200) for 1 h at RT. Following three washes with PBS, coverslips were mounted on slides using Prolong Gold (with DAPI).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Rab5a Antibody (NBP1-58880) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Rab5a Antibody (NBP1-58880)

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Blogs on Rab5a

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Contact Information

Product PDFs

Calculators

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

NIKHIL VAD
07/17/2018
Application: Immunofluorescence (IF)
Species: Human

Bioinformatics

Gene Symbol RAB5A
Uniprot