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PRRG2 Antibody

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Western Blot: PRRG2 Antibody [NBP1-87229] - Analysis in control (vector only transfected HEK293T lysate) and PRRG2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PRRG2 Antibody [NBP1-87229] - Immunofluorescent staining of human cell line RT4 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: PRRG2 Antibody [NBP1-87229] - Staining of human heart muscle shows strong membranous positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PRRG2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QEVFLGPPEAQSFLSSHTRIPRANHWDLELLTPGNLERECLEERCSWEEAREYFEDNTLTERFWESYIYNGKGGRGRVDVA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRRG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PRRG2 Protein (NBP1-87229PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PRRG2 Antibody

  • PRGP2proline-rich Gla (G-carboxglutamic acid) polypeptide 2
  • proline rich Gla (G-carboxyglutamic acid) 2
  • Proline-rich gamma-carboxyglutamic acid protein 2
  • proline-rich Gla (G-carboxyglutamic acid) polypeptide 2
  • Proline-rich Gla protein 2
  • TMG2
  • transmembrane gamma-carboxyglutamic acid protein 2

Background

PRRG2, also known as Proline Rich Gla (G-Carboxyglutamic Acid) 2, is a 179 amino acid protein that is 20 kDa. Little is currently known about its function. This protein is being studied for its involvement in thyroiditis. The PRRG2 protein has also shown an interaction with NEDD4, PXN and VCL proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRRG2