Protocadherin-15 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Protocadherin 15 (PCDH-15) is a 250-270 kDa "other" member of the nonclustered group of the protocadherin family of molecules. In the adult, it is expressed on the base of hair cell stereoclila in the Organ of Corti, on cerebellar granule cells, and on rod and cone photoreceptors. In the ear, it is expressed as a homodimer, and binds (in trans) to cadherin 23 homodimers on adjacent stereocilia. Mature human PCDH-15 is a type I transmembrane protein that is 1929 amino acids (aa) in length. It contains a 1350aa extracellular domain (ECD) (aa 27-1376) plus a 558 aa cytoplasmic region. There are 11 cadherin domains in the ECD (aa 40-1259). Multiple splice forms are reported. One is a soluble 110 kDa form that shows a five aa substitution for aa 957-1955. Another contains a seven aa substitution for aa 1119-1125. Others show substitutions or deletions in either the extracellular or cytoplasmic domains. Over aa 27-1376 (the ECD), human PCDH-15 shares 95% aa identity with mouse PCDH-15.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Primate, Xenopus.

We have publications tested in 2 applications: IHC, IHC-Fr.

Showing Publications 1 - 4 of 4.

Publications using AF6729	Applications	Species
Ivanchenko, MV;Hathaway, DM;Mulhall, EM;Booth, KT;Wang, M;Peters, CW;Klein, AJ;Chen, X;Li, Y;György, B;Corey, DP; PCDH15 Dual-AAV Gene Therapy for Deafness and Blindness in Usher Syndrome Type 1F bioRxiv : the preprint server for biology 2023-11-13 [PMID: 38014037] (IHC, Primate)	IHC	Primate
Verschueren A, Boucherit L, Ferrari U et al. Planar polarity in primate cone photoreceptors: a potential role in Stiles Crawford effect phototropism Communications Biology 1/24/2022 [PMID: 35075261]
Wei Huang, Aparna Bhaduri, Dmitry Velmeshev, Shaohui Wang, Li Wang, Catherine A. Rottkamp et al. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells Cell 8/1/2020 [PMID: 32679030]
C Schietroma, K Parain, A Estivalet, A Aghaie, J Boutet de, S Picaud, JA Sahel, M Perron, A El-Amraoui, C Petit Usher syndrome type 1-associated cadherins shape the photoreceptor outer segment J. Cell Biol., 2017-05-11;0(0):. 2017-05-11 [PMID: 28495838] (IHC-Fr, Xenopus)	IHC-Fr	Xenopus

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/02/2018	View Summary Application Western Blot Sample Tested HEK 293 transfected,HEK293 transfected cells Species Human Lot 1806A Comments Comments PVDF membrane was blocked with 3% milk for 1 hour at room temperature. Primary antibody sheep anti-Pcdh15 was used at a concentration 2ug/mL in 3% milk and the membrane incubated overnight at 4C. Secondary antibody anti-sheep-HRP was used at a dilution 1:2500 in 3% milk and incubated for 1 hour at room temperature.
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Mouse	09/25/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested Organ of Corti (inner ear) Species Mouse Lot 1806A Comments Comments P1 mouse organ of Corti was paraformaldehyde fixed (4%) for 30 min, blocked 1 hour in PBS Triton X-100 0.1% containing 10% fetal calf serum and then incubated overnight with sheep anti-Pcdh15 (1:200, green) in blocking solution. Secondary antibody was Alexa488 anti-sheep (1:200). Tissue was counterstained with phalloidin (red).

Array AF6729

• Protocadherin-15 Antibodies
• Protocadherin-15 ELISA Kits
• Protocadherin-15 Lysate