Podocalyxin Like Antibody (222328) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Virus.

We have publications tested in 9 applications: Cell Selection, ELISA Capture, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Immunoprecipitation.

Showing Publications 1 - 10 of 22. Show All 22 Publications. Collapse Publications.

Publications using MAB1658	Applications	Species
Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse)	Immunohistochemistry	Human, Mouse
Rakotomamonjy, J;Rylaarsdam, L;Fares-Taie, L;McDermott, S;Davies, D;Yang, G;Fagbemi, F;Epstein, M;Fairbanks-Santana, M;Rozet, JM;Guemez-Gamboa, A; PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model Cell reports 2023-07-20 [PMID: 37480564] (ICC, Human)	ICC	Human
Huang B, Zeng Z, Li H et al. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids bioRxiv 2023-05-26 [PMID: 37293038]
Huang B, Zeng Z, Li H et al. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids bioRxiv 5/26/2023 [PMID: 37293038]
Laura Amo, Javier Díez-García, Estíbaliz Tamayo-Orbegozo, Natalia Maruri, Susana Larrucea Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site Frontiers in Immunology 5/31/2022 [PMID: 35711462]
R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis Cell Stem Cell, 2021-11-29;0(0):. 2021-11-29 [PMID: 34847364] (IHC, Human)	IHC	Human
Matteo A. Molè, Tim H. H. Coorens, Marta N. Shahbazi, Antonia Weberling, Bailey A. T. Weatherbee, Carlos W. Gantner et al. A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre Nature Communications 6/17/2021 [PMID: 34140473]
MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration Science Advances, 2021-05-19;7(21):. 2021-05-19 [PMID: 34138734] (Flow Cytometry, Human)	Flow Cytometry	Human
D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu Identification of the SHREK Family of Proteins as Broad-Spectrum Host Antiviral Factors Viruses, 2021-05-04;13(5):. 2021-05-04 [PMID: 34064525] (Immunoprecipitation, Virus)	Immunoprecipitation	Virus
A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo Naive stem cell blastocyst model captures human embryo lineage segregation Cell Stem Cell, 2021-05-05;28(6):1016-1022.e4. 2021-05-05 [PMID: 33957081] (ICC, Human)	ICC	Human
D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu Identification of the SHREK family of proteins as broad-spectrum host antiviral factors bioRxiv : the preprint server for biology, 2021-02-03;0(0):. 2021-02-03 [PMID: 33564770] (ELISA Capture, ICC, Human)	ELISA Capture, ICC	Human
Ryan F. Townshend, Yue Shao, Sicong Wang, Chari L. Cortez, Sajedeh Nasr Esfahani, Jason R. Spence et al. Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Frontiers in Cell and Developmental Biology 10/15/2020 [PMID: 33178701]
E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells Oncotarget, 2017-09-27;8(59):99722-99739. 2017-09-27 [PMID: 29245936] (Flow Cytometry, Human)	Flow Cytometry	Human
Self-organization of the human embryo in the absence of maternal tissues Nat. Cell Biol., 2016-05-04;18(6):700-8. 2016-05-04 [PMID: 27144686] (IHC, Human)	IHC	Human
Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome. PLoS ONE, 2013-10-16;8(10):e75945. 2013-10-16 [PMID: 24146797] (IHC-P, Human)	IHC-P	Human
Lasagni L, Ballerini L, Angelotti ML Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders. Stem Cells, 2010-09-01;28(9):1674-85. 2010-09-01 [PMID: 20680961] (IHC-Fr, Human)	IHC-Fr	Human
MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 2020-08-10;11(1):3987. 2020-08-10 [PMID: 32778678] (ICC, IHC, Human)	ICC, IHC	Human
Kenichiro Taniguchi, Yue Shao, Ryan F. Townshend, Chari L. Cortez, Clair E. Harris, Sasha Meshinchi et al. An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells Journal of Cell Biology 12/4/2017 [PMID: 29021220]
Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury. Stem Cells, 2012-08-01;30(8):1714-25. 2012-08-01 [PMID: 22628275] (Flow Cytometry, Human)	Flow Cytometry	Human
David T. Madden, Diana Davila-Kruger, Simon Melov, Dale E. Bredesen Human Embryonic Stem Cells Express Elevated Levels of Multiple Pro-Apoptotic BCL-2 Family Members PLoS ONE 12/9/2011 [PMID: 22174832]
Ronconi E, Sagrinati C, Angelotti ML Regeneration of glomerular podocytes by human renal progenitors. J. Am. Soc. Nephrol., 2008-12-17;20(2):322-32. 2008-12-17 [PMID: 19092120] (Cell Selection, Human)	Cell Selection	Human
Show All 22 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	Simple Western	Human	08/21/2017	View Summary Application Simple Western Sample Tested lysates from melanoma cells Species Human Lot JKW0217021 Comments Comments The monoclonal mouse antibody, MAB 1658 yields a single sharp peak at 56 kda, corresponding to the expected size for PODXL. The signal is medium strong, as evident from the y-axis. The signal is identical in cell and EV lysates, but absent in the exosomes (this exo lysate has produced results with other antibodies). I used a regular 12-230 kDa matrix and standard settings. Both antibodies were used at a dilution of 1:50. The lysates were at a concentration of 0,6 ug/ul

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• Podocalyxin Like Antibodies
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• Podocalyxin Like Proteins
• Podocalyxin Like Proteins and Enzymes