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PKA RI beta Antibody (OTI2A3)

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Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA regulatory subunit I beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKA regulatory subunit I beta.
Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA regulatory subunit I beta (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA regulatory subunit I beta.
Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKA regulatory subunit I beta mouse monoclonal antibody.
Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PKA regulatory subunit I ...read more
Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of paraffin-embedded Human lymph node tissue using anti-PKA regulatory subunit I beta mouse monoclonal antibody.
Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Analysis of Hela cells, using anti-PKA regulatory subunit I beta antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Analysis of Jurkat cells, using anti-PKA regulatory subunit I beta antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.57 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73430AF350
Alexa Fluor 405
NBP2-73430AF405
Alexa Fluor 488
NBP2-73430AF488
Alexa Fluor 532
NBP2-73430AF532
Alexa Fluor 594
NBP2-73430AF594
Alexa Fluor 647
NBP2-73430AF647
Alexa Fluor 700
NBP2-73430AF700
Alexa Fluor 750
NBP2-73430AF750
Allophycocyanin
NBP2-73430APC
Allophycocyanin/Cy7
NBP2-73430APCCY7
Biotin
NBP2-73430B
CoraFluor 1
NBP2-73430CL1
DyLight 350
NBP2-73430UV
DyLight 405
NBP2-73430V
DyLight 488
NBP2-73430G
DyLight 550
NBP2-73430R
DyLight 594
NBP2-73430DL594
DyLight 650
NBP2-73430C
DyLight 680
NBP2-73430FR
DyLight 755
NBP2-73430IR
FITC
NBP2-73430F
HRP
NBP2-73430H
Janelia Fluor 525
NBP2-73430JF525
Janelia Fluor 549
NBP2-73430JF549
Janelia Fluor 585
NBP2-73430JF585
Janelia Fluor 635
NBP2-73430JF635
Janelia Fluor 646
NBP2-73430JF646
Janelia Fluor 669
NBP2-73430JF669
PE
NBP2-73430PE
PE/Atto594
NBP2-73430PEATT594
PE/Cy5.5
NBP2-73430PECY55
PE/Cy7
NBP2-73430PECY7
PerCP
NBP2-73430PCP
mFluor Violet 450 SE
NBP2-73430MFV450
mFluor Violet 500 SE
NBP2-73430MFV500
mFluor Violet 610 SE
NBP2-73430MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73430
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PKA RI beta Antibody (OTI2A3) Summary

Immunogen
Full length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAR1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.57 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKA RI beta Antibody (OTI2A3)

  • PKA RI beta
  • PRKAR1
  • PRKAR1B
  • RIbeta

Background

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PRKAR1B
Entrez