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PITRM1 Antibody

Images

 
Independent Antibodies: Western Blot: PITRM1 Antibody [NBP1-81729] - Analysis using Anti-PITRM1 antibody NBP1-81729 (A) shows similar pattern to independent antibody NBP1-81728 (B).
Immunocytochemistry/ Immunofluorescence: PITRM1 Antibody [NBP1-81729] - Staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human cerebral cortex.
Western Blot: PITRM1 Antibody [NBP1-81729] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human salivary gland shows low expression as expected.
Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining in human testis and salivary gland tissues using anti-PITRM1 antibody. Corresponding PITRM1 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human cerebral cortex, colon, heart muscle and testis using Anti-PITRM1 antibody NBP1-81729 (A) shows similar ...read more
Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human heart muscle.
Immunohistochemistry-Paraffin: PITRM1 Antibody [NBP1-81729] - Staining of human colon.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PITRM1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ELRSQQSKPQDASCLPALKVSDIEPTIPVTELDVVLTAGDIPVQYCAQPTNGMVYFRAFSSLNTLPEELRPYVPLFCSVLTKLGCGLLDYREQAQQIELKTGGMSASPHVLPDDSHMDTYEQGVLFSSLCLDRNLPDMMQLWSEI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PITRM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PITRM1 Protein (NBP1-81729PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PITRM1 Antibody

  • EC 3.4.24
  • EC 3.4.24.-
  • hPreP
  • Metalloprotease 1
  • MGC138192
  • MGC141929
  • pitrilysin metallopeptidase 1
  • Pitrilysin metalloproteinase 1mitochondrial
  • PreP

Background

ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PITRM1