![ELISA: Human Perforin ELISA Kit (Colorimetric) [NBP3-00418] - Samples were spiked with high concentrations of Human Perforin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00418_human-perforin-elisa-kit-colorimetric-132202416173413.jpg "ELISA: Human Perforin ELISA Kit (Colorimetric) [NBP3-00418] - Samples were spiked with high concentrations of Human Perforin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma, cell supernatant, and other biological fluids |
| Standard Curve Range | 0.78 - 50 ng/mL |
| Sensitivity | 0.47 ng/mL |
| Specificity | This kit recognizes Human Perforin in samples. No significant cross-reactivity or interference between Human Perforin and analogues was observed. |
| Standard Curve Range | 0.78 - 50 ng/mL |
| Sensitivity | 0.47 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | 4.6% |
| Intra-Assay | 4.81% |
| Spike Recovery | 87 - 107% |
| Sample Volume | 100 ul |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | PRF1 |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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Perforin a Protective Serial Killer Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy... Read full blog post. |
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Perforin Antibodies Reveal Links to Apoptosis and Immune Response Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel... Read full blog post. |
| Gene Symbol | PRF1 |
![ELISA: Human Perforin ELISA Kit (Colorimetric) [NBP3-00418] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00418_human-perforin-elisa-kit-colorimetric-4320249192898.jpg "ELISA: Human Perforin ELISA Kit (Colorimetric) [NBP3-00418] - Standard Curve Reference")
