![Western Blot: PCTP Antibody (1F9) [H00058488-M01] - PCTP monoclonal antibody (M01), clone 1F9 Analysis of PCTP expression in K-562.](http://images.novusbio.com/fullsize/PCTP-Antibody-1F9-Western-Blot-H00058488-M01-img0007.jpg "Western Blot: PCTP Antibody (1F9) [H00058488-M01] - PCTP monoclonal antibody (M01), clone 1F9 Analysis of PCTP expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 1F9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | PCTP (AAH12084, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVLEDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQRRDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPGGQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT |
| Localization | Cytoplasmic |
| Specificity | PCTP - phosphatidylcholine transfer protein |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PCTP |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: PCTP Antibody (1F9) [H00058488-M01] - Analysis of PCTP over-expressed 293 cell line, cotransfected with PCTP Validated Chimera RNAi ( Cat # H00058488-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCTP monoclonal antibody (M01), clone 1F9 (Cat # H00058488-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PCTP-Antibody-1F9-Western-Blot-H00058488-M01-img0005.jpg "Western Blot: PCTP Antibody (1F9) [H00058488-M01] - Analysis of PCTP over-expressed 293 cell line, cotransfected with PCTP Validated Chimera RNAi ( Cat # H00058488-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCTP monoclonal antibody (M01), clone 1F9 (Cat # H00058488-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: PCTP Antibody (1F9) [H00058488-M01] - Analysis of PCTP expression in transfected 293T cell line by PCTP monoclonal antibody (M01), clone 1F9.Lane 1: PCTP transfected lysate(24.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCTP-Antibody-1F9-Western-Blot-H00058488-M01-img0008.jpg "Western Blot: PCTP Antibody (1F9) [H00058488-M01] - Analysis of PCTP expression in transfected 293T cell line by PCTP monoclonal antibody (M01), clone 1F9.Lane 1: PCTP transfected lysate(24.8 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: PCTP Antibody (1F9) [H00058488-M01] - Detection limit for recombinant GST tagged PCTP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PCTP-Antibody-1F9-Sandwich-ELISA-H00058488-M01-img0006.jpg "Sandwich ELISA: PCTP Antibody (1F9) [H00058488-M01] - Detection limit for recombinant GST tagged PCTP is approximately 0.03ng/ml as a capture antibody.")
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