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PAX8 Antibody (CL7834)

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Staining of human Fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Staining of human Fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.
Staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.
Analysis in human cell line EFO-21.
Staining of EFO-21 cells using the Anti-PAX8 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL7834
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PAX8 Antibody (CL7834) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of Q06710. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PAX8
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for PAX8 Antibody (CL7834)

  • paired box 8
  • paired box gene 8
  • paired box protein Pax-8
  • paired domain gene 8

Background

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAX8