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Patched 2/PTCH2 Antibody (OTI8B5)

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SDS-Page: Patched 2/PTCH2 Antibody (OTI8B5) [NBP2-45440] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH2.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, PAGE
Clone
OTI8B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Patched 2/PTCH2 Antibody (OTI8B5) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 79-392 of human PTCH2 (NP_003729) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTCH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • SDS-Page
  • Western Blot 1:2000
Theoretical MW
130.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Patched 2/PTCH2 Antibody (OTI8B5)

  • patched (Drosophila) homolog 2
  • Patched 2
  • patched homolog 2 (Drosophila)
  • patched homolog 2
  • protein patched homolog 2
  • ptc2
  • PTCH2

Background

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas. PTCH2 has a frameshift mutation resulting in a truncated PTCH2 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional Patched 2/PTCH2 Products

Array NBP2-45440

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Bioinformatics

Gene Symbol PTCH2