Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC |
Clone | SX53G8 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34718) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen | Purified GST-p27 fusion protein of human origin (Uniprot: P46527) |
Localization | Nuclear |
Specificity | This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | CDKN1B |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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Publications |
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Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Publication using NBP2-32889 | Applications | Species |
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Gao J, Wu L, Wang Y et al. Knockdown of Cxcl10 Inhibits Mesangial Cell Proliferation in Murine Habu Nephritis Via ERK Signaling Cell. Physiol. Biochem. 2017-08-15 [PMID: 28810249] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for p27/Kip1 Antibody (NBP2-32889)Find related products by research area.
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