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Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

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ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] - Samples were spiked with high concentrations of Rat p21/CIP1/CDKN1A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] - Samples were spiked with high concentrations of Rat p21/CIP1/CDKN1A and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CDKN1A in samples. No significant cross-reactivity or interference between Rat CDKN1A and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.92%
Intra-Assay
CV% < 5%
Spike Recovery
90-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CDKN1A

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-75207.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol CDKN1A