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Mouse p19ARF/CDKN2A ELISA Kit (Colorimetric)

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ELISA: Mouse p19ARF/CDKN2A ELISA Kit (Colorimetric) [NBP3-38956] - Standard Curve Reference
ELISA: Mouse p19ARF/CDKN2A ELISA Kit (Colorimetric) [NBP3-38956] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.108 ng/mL (example only; lot dependent)

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Mouse p19ARF/CDKN2A ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.108 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
Cdkn2a

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Mouse p19ARF/CDKN2A ELISA Kit (Colorimetric)

  • alternative reading frame
  • ARF
  • ARF-INK4a
  • CDK4I
  • CDKN2A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • cyclin-dependent kinase inhibitor 2A, isoform 3
  • cyclin-dependent kinase inhibitor 2A, isoforms 1/2
  • cyclin-dependent kinase inhibitor protein
  • INK4
  • INK4a
  • Ink4a/Arf
  • INK4a-ARF
  • mitochondrial smARF'
  • MTS1
  • p14
  • p14ARF
  • P16
  • p16(INK4a)
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • p19<ARF>
  • p19ARF
  • Pctr1

Background

p19ARF (mouse) and p14ARF (human) has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol Cdkn2a