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p14ARF/CDKN2A Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-159AF350
Alexa Fluor 405
NB200-159AF405
Alexa Fluor 488
NB200-159AF488
Alexa Fluor 594
NB200-159AF594
Alexa Fluor 647
NB200-159AF647
Alexa Fluor 700
NB200-159AF700
Alexa Fluor 750
NB200-159AF750
Allophycocyanin
NB200-159APC
Biotin
NB200-159B
CoraFluor 1
NB200-159CL1
DyLight 350
NB200-159UV
DyLight 405
NB200-159V
DyLight 488
NB200-159G
DyLight 594
NB200-159DL594
DyLight 650
NB200-159C
DyLight 680
NB200-159FR
DyLight 755
NB200-159IR
FITC
NB200-159F
HRP
NB200-159H
Janelia Fluor 525
NB200-159JF525
Janelia Fluor 549
NB200-159JF549
Janelia Fluor 646
NB200-159JF646
Janelia Fluor 669
NB200-159JF669
PE
NB200-159PE
PerCP
NB200-159PCP
Unconjugated
NB200-159
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p14ARF/CDKN2A Antibody [DyLight 550] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
The epitope recognized by this antibody maps to a region between residue 82 and C-terminus (aa132) of human p14ARF using the numbering given in entry Uniprot ID Q8N726.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN2A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry-Paraffin
Application Notes
This p14ARF antibody is useful for ELISA, Immunohistochemistry on paraffin-embedded sections and Western blot. In WB a band is seen at ~14 kDa representing p14ARF. *The investigator should determine the optimal working dilution for a specific application.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Based on 100% sequence identity, this antibody is predicted to react with Orangutan and White-tufted-ear marmoset.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for p14ARF/CDKN2A Antibody [DyLight 550]

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Background

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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p14ARF/CDKN2A
Antibody [DyLight 550
NB200-159R
p14ARF/CDKN2A Antibody [DyLight 550]
Species: Hu, Pm
Applications: ELISA, IHC

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Bioinformatics

Gene Symbol CDKN2A