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p14ARF/CDKN2A Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-158AF350
Alexa Fluor 405
NB200-158AF405
Alexa Fluor 488
NB200-158AF488
Alexa Fluor 532
NB200-158AF532
Alexa Fluor 594
NB200-158AF594
Alexa Fluor 647
NB200-158AF647
Alexa Fluor 700
NB200-158AF700
Alexa Fluor 750
NB200-158AF750
Allophycocyanin
NB200-158APC
Biotin
NB200-158B
DyLight 350
NB200-158UV
DyLight 405
NB200-158V
DyLight 488
NB200-158G
DyLight 550
NB200-158R
DyLight 594
NB200-158DL594
DyLight 650
NB200-158C
DyLight 680
NB200-158FR
DyLight 755
NB200-158IR
FITC
NB200-158F
HRP
NB200-158H
Janelia Fluor 525
NB200-158JF525
Janelia Fluor 549
NB200-158JF549
Janelia Fluor 585
NB200-158JF585
Janelia Fluor 635
NB200-158JF635
Janelia Fluor 646
NB200-158JF646
Janelia Fluor 669
NB200-158JF669
PE
NB200-158PE
PerCP
NB200-158PCP
Unconjugated
NB200-158
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p14ARF/CDKN2A Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
The epitope recognized by this antibody maps to a region between residue 25 and 75 of human p14ARF using the numbering given in entry Uniprot ID Q8N726.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN2A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for p14ARF/CDKN2A Antibody [CoraFluor™ 1]

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Background

P14ARF is an alternate reading frame (ARF) product of the CDKN2A locus, which acts as a tumor suppressor by inducing cell cycle arrest in G1 and G2 phases and apoptosis.

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded by differential splicing of alternative first exons. The p16INK4a (exon 1 alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF protein complexes with MDM2 within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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p14ARF/CDKN2A
Antibody [CoraFluor&# ...
NB200-158CL1
p14ARF/CDKN2A Antibody [CoraFluor™ 1]
Species: Hu
Applications: WB, ELISA, IHC

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Bioinformatics

Gene Symbol CDKN2A