![Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: ~1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: ~1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block buffer overnight at 4C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin.](http://images.novusbio.com/fullsize/Ovalbumin Antibody [Biotin]-Western Blot-NB600-924-img0005.jpg "Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: ~1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: ~1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block buffer overnight at 4C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin.")
| Reactivity | ChSpecies Glossary |
| Applications | WB, DB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NB120-20546-0.1mg | |||
| Unconjugated | NB600-922-0.1mg | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White] |
| Immunogen | Ovalbumin [Hen Egg White] (Uniprot: P01012) |
| Localization | Secreted |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | OVAL |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below. |
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| Readout System | ||
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| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.](http://images.novusbio.com/fullsize/Ovalbumin-Antibody-[Biotin]-Western-Blot-NB600-924-img0004.jpg "Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")