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Otolin-1 Antibody (839418) [Unconjugated]

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Western blot shows lysates of BG01V human embryonic stem cells undifferentiated or differentiated to early otic lineage. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Otolin-1 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of BG01V human embryonic stem cells undifferentiated or differentiated to early otic lineage. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Otolin-1 Monoclonal Antibody ...read more
Otolin-1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to early otic lineage using Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
839418
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8045-SP
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Otolin-1 Antibody (839418) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Otolin-1
Lys24-Pro477
Accession # A6NHN0
Specificity
Detects human Otolin-1 in ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
OTOL1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otolin-1 Antibody (839418) [Unconjugated]

  • C1q and tumor necrosis factor related protein 15
  • C1qTNF15
  • LOC131149
  • OTOL1
  • otolin 1
  • Otolin-1

Background

Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear. It is secreted by supporting cells of the sensory epithelium. The otoconial membrane contains particles known as otoconia which are composed of glycoproteins and proteoglycans coated with calcium carbonate crystals. Otolin is one of the protein components of otoconia particles, and it is important for otoconia formation as well as for auditory and vestibular function. It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including Cerebellin-1 and Otoconin-90. Otolin contains three collagen-like regions followed by a C1q-like domain at the C-terminus. It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions.  Human Otolin shares 72% aa sequence identity with mouse and rat Otolin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol OTOL1
Uniprot