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Optimedin Antibody (3A2)

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Western Blot: Optimedin Antibody (3A2) [H00118427-M06] - Analysis of OLFM3 expression in Raw 264.7 (Cat # L024V1).
Western Blot: Optimedin Antibody (3A2) [H00118427-M06] - Analysis of OLFM3 expression in PC-12 (Cat # L012V1).
Western Blot: Optimedin Antibody (3A2) [H00118427-M06] - Analysis of OLFM3 expression in NIH/3T3 (Cat # L018V1).
ELISA: Optimedin Antibody (3A2) [H00118427-M06] - Detection limit for recombinant GST tagged OLFM3 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clone
3A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Optimedin Antibody (3A2) Summary

Immunogen
OLFM3 (NP_477518, 108 a.a. ~ 206 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FRQIEDDRKTLMTKHFQELKEKMDELLPLIPVLEQYKTDAKLITQFKEEIRNLSAVLTGIQEEIGAYDYEELHQRVLSLETRLRDCMKKLTCGKLMKIT
Specificity
OLFM3 - olfactomedin 3
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OLFM3
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Optimedin Antibody (3A2)

  • NOE3OPTIMEDIN
  • noelin 3
  • NOELIN3
  • noelin-3
  • NOELIN3_V1
  • NOELIN3_V2
  • NOELIN3_V3
  • NOELIN3_V4
  • NOELIN3_V5
  • NOELIN3_V6
  • olfactomedin 3
  • olfactomedin related ER localized protein 3
  • olfactomedin-3
  • Optimedin

Background

Optimedin, also known as olfactomedin 3 (OLFM3) and Noelin-3, is a protein expressed in the eyes, brain, and lungs. The protein is known to stimulate the formation of cell adherent and cell-cell tight junctions. Additionally, its expression modulates cell migration and the organization of the cytoskeleton. In one study, cells expressing high levels of optimedin showed higher growth rates and stronger adhesion to the collagen extracellular matrix as compared with control cells. Optimedin has also been identified as a candidate gene for glaucoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OLFM3
Entrez
OMIM
Uniprot