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NMDA R, NR1 Subunit Antibody [Unconjugated] - C1 Splice Variant

Images

 
Western blot of HEK 293 cells: Lane 1 - HEK Cells without NR1 expression (Mock); Lane 2 - NR1 subunit containing only the C2 Splice Variant; Lane 3 - NR1 subunit containing the C1 and C2' splice variants; Lane 4 - NR1 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NMDA R, NR1 Subunit Antibody [Unconjugated] - C1 Splice Variant Summary

Immunogen
Peptide from the NR1 subunit, C1 Splice Variant of the NMDA Receptor
Specificity
Mouse and rat ~120 kDa NR1 subunit of the NMDA Receptor containing the C1 splice variant
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
Grin1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2000 dilution
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Buffer
25 μg lyophilized in 5 mM ammonium bicarbonate.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute with 50 μL of sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR1 Subunit Antibody [Unconjugated] - C1 Splice Variant

  • GluN1
  • GRIN1
  • MRD8
  • NDHMSD
  • NDHMSR;NMD-R1
  • NMDA R, NR1 Subunit
  • NMDA1
  • NMDAR1
  • NMD-R1
  • NR1

Background

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor is a heteromultimer of at least two NR1 (NMDA receptor-1) subunits and two NR2 subunits. Regulation of the NMDA complex by external factors such as zinc, PKC and polyamines is mediated by NR1 subunits. Upon glycine binding to NR1 and glutamate binding to NR2, the NMDA channel is opened allowing calcium and sodium influx into the cell. NR1 is a 120 kDa, 938 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 541 aa extracellular domain and a 105 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. Differential splicing of three exons generates up to nine isoforms of NR1. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Eight are integral membrane proteins. At the N-terminus, four isoforms show a 21 aa insertion between aa 190 - 191, and four do not. This is compounded by at least four possible variants between aa 864 - 938 in the cytoplasmic tail that impact potential serine phosphorylation sites. To date, all are believed to be physiologically active. There is one potentially “soluble” form that is only 181 aa in length. Its function is unknown.
  1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
  2. Sugihara, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:826.
  3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
  4. Moriyoshi, K. et al. (1991) Nature 354:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol Grin1