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NLRP3/NALP3 Antibody (768319) [Alexa Fluor® 488]

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RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7578G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7578G, filled histogram) or isotype control antibody ...read more
Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7578G, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
768319
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC7578U-100UG
Alexa Fluor 532
FAB7578X
Alexa Fluor 594
IC7578T-100UG
Alexa Fluor 647
IC7578R-100UG
Alexa Fluor 700
IC7578N
Alexa Fluor 750
IC7578S-100UG
Allophycocyanin
IC7578A
Biotin
FAB7578B
CoraFluor 1
FAB7578CL1
DyLight 350
FAB7578D
DyLight 405
FAB7578E
DyLight 488
FAB7578K
DyLight 550
FAB7578L
DyLight 594
FAB7578M
DyLight 650
FAB7578W
DyLight 680
FAB7578Y
DyLight 755
FAB7578Z
FITC
FAB7578F
HRP
FAB7578H
Janelia Fluor 525
FAB7578JF525
Janelia Fluor 549
FAB7578I
Janelia Fluor 646
FAB7578J
Janelia Fluor 669
FAB7578JF669
Phycoerythrin
IC7578P
Unconjugated
MAB7578
mFluor Violet 450 SE
FAB7578MFV450
mFluor Violet 500 SE
FAB7578MFV500
mFluor Violet 610 SE
FAB7578MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB7578
Carrier Free
IC7578P
Carrier Free
MAB7578
Carrier Free
IC7578A
Carrier Free
IC7578N
Carrier Free
MAB7578-SP
Carrier Free
IC7578U-100UG
Carrier Free
IC7578T-100UG
Carrier Free
IC7578S-100UG
Carrier Free
IC7578R-100UG
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NLRP3/NALP3 Antibody (768319) [Alexa Fluor® 488] Summary

Immunogen
E. coli-derived recombinant mouse NLRP3/NALP3
Met1-Arg153
Accession # Q8R4B8
Specificity
Detects mouse NLRP3/NALP3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
NLRP3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells
Publications
Read Publications using
IC7578G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NLRP3/NALP3 Antibody (768319) [Alexa Fluor® 488]

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NLRP3/NALP3 Antibody (IC7578G)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(3)
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FAQs for NLRP3/NALP3 Antibody (IC7578G). (Showing 1 - 4 of 4 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • An E. coli derived sequence corresponding to Met1 - Arg153 of mouse NLRP3/NALP3.

Secondary Antibodies

 

Isotype Controls

Additional NLRP3/NALP3 Products

Blogs on NLRP3/NALP3.

Check out the latest blog posts on NLRP3/NALP3.
The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol NLRP3
Uniprot