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NIPP1 Antibody (OTI4E5) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

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NIPP1 Antibody (OTI4E5) [DyLight 350] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 1-225 of human NIPP1 (NP_612568) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPP1R8
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for NIPP1 Antibody (OTI4E5) [DyLight 350]

  • activator of RNA decay
  • ARD1
  • ard-1
  • ARD1nuclear inhibitor of protein phosphatase 1
  • NIPP1
  • NIPP1nuclear inhibitor of protein phosphatase-1
  • NIPP-1nuclear subunit of PP-1
  • PPP1R8
  • PRO2047
  • Protein phosphatase 1 regulatory inhibitor subunit 8
  • protein phosphatase 1 regulatory subunit 8
  • protein phosphatase 1, regulatory (inhibitor) subunit 8
  • RNase E

Background

NIPP1, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP1R8