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Recombinant Human NCK1 His Protein

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SDS-Page: NCK1 Protein [NBP1-45253] - 15% SDS-PAGE (3ug)
SDS-Page: NCK1 Protein [NBP1-45253] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human NCK1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-377 of Human NCK1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NCK1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 1 mM EDTA, 50 mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human NCK1 His Protein

  • cytoplasmic protein NCK1
  • MGC12668
  • NCK adaptor protein 1melanoma NCK protein
  • NCK1
  • nck-1
  • NCKalpha
  • NCKNCK tyrosine kinase
  • non-catalytic region of tyrosine kinase
  • SH2/SH3 adaptor protein NCK-alpha

Background

NCK adaptor protein 1, also known as NCK, belongs to the adaptor family of proteins. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast. Recombinant human NCK1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NCK1