Novus Biologicals products are now on bio-techne.com

NAP1L1 Overexpression Lysate

Images

 
Western Blot: NAP1L1 Overexpression Lysate (Adult Normal) [NBL1-13469] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAP1L1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NAP1L1 Overexpression Lysate Summary

Description

NAP1L1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC220821

Accession#: NM_004537

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
NAP1L1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for NAP1L1 Overexpression Lysate

  • hNRP
  • HSP22-like protein interacting protein
  • MGC23410
  • MGC8688
  • NAP-1 related protein
  • NAP1
  • NAP1LFLJ16112
  • NRPNAP-1-related protein
  • nucleosome assembly protein 1-like 1

Background

This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing of this gene results in several transcript variants; however, not all have been fully described. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF566
Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
DVE00
Species: Hu
Applications: ELISA
AF567
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
NBP3-30941
Species: Hu, Rt
Applications: IHC, WB
AF7109
Species: Mu
Applications: IHC, WB
NBP1-82527
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-36543
Species: Hu
Applications: IP, WB
AF5514
Species: Hu, Mu
Applications: WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
H00010097-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
H00008337-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
AF1444
Species: Mu
Applications: IHC, WB
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-07996
Species: Hu
Applications: WB
NBP2-52486
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP3-03514
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NLS3775
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for NAP1L1 Lysate (NBL1-13469) (0)

There are no publications for NAP1L1 Lysate (NBL1-13469).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NAP1L1 Lysate (NBL1-13469) (0)

There are no reviews for NAP1L1 Lysate (NBL1-13469). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NAP1L1 Lysate (NBL1-13469) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NAP1L1 Products

Research Areas for NAP1L1 Lysate (NBL1-13469)

Find related products by research area.

Blogs on NAP1L1

There are no specific blogs for NAP1L1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NAP1L1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NAP1L1