![Western Blot: Myosin 5a Antibody [NBP1-49858] - Analysis of Myosin 5a in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Western-Blot-NBP1-49858-img0001.jpg "Western Blot: Myosin 5a Antibody [NBP1-49858] - Analysis of Myosin 5a in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ETKQLELDLN corresponding to internal region according to NP_001135967.1. |
| Epitope | ETKQLELDLN - internal region |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1). |
| Predicted Species | Rat (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | MYO5A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IF: Transverse sections of mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodiesscientific literature (PMID: 20133847). WB: Approx. 200 kDa band observed in Jurkat lysates (calculated MW of 215 kDa band according to NP_000250.3). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Myosin 5a Antibody (NBP1-49858)Find related products by research area.
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| Gene Symbol | MYO5A |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-49858] - (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rudiger Rudolf, Karlsruhe, Germany](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49858-img0005.jpg "Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-49858] - (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rudiger Rudolf, Karlsruhe, Germany")
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