![Western Blot: MTAP Antibody [NBP1-32718] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:5000.](http://images.novusbio.com/fullsize/MTAP-Antibody-Western-Blot-NBP1-32718-img0009.jpg "Western Blot: MTAP Antibody [NBP1-32718] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:5000.")
| Reactivity | Hu, Mu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human MTAP Recombinant protein. |
| Localization | Cytoplasm |
| Predicted Species | Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MTAP |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MTAP |
![Immunocytochemistry/Immunofluorescence: MTAP Antibody [NBP1-32718] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32718-img0007.jpg "Immunocytochemistry/Immunofluorescence: MTAP Antibody [NBP1-32718] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718] - SAS xenograft , using MTAP antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunohistochemistry-Paraffin-NBP1-32718-img0003.jpg "Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718] - SAS xenograft , using MTAP antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: MTAP Antibody [NBP1-32718] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MTAP-Antibody-Western-Blot-NBP1-32718-img0004.jpg "Western Blot: MTAP Antibody [NBP1-32718] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunoprecipitation: MTAP Antibody [NBP1-32718] - Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 ug of MTAP antibody [N1C3]. Western blot analysis was performed using MTAP antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunoprecipitation-NBP1-32718-img0008.jpg "Immunoprecipitation: MTAP Antibody [NBP1-32718] - Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 ug of MTAP antibody [N1C3]. Western blot analysis was performed using MTAP antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Binding Activity IFN-beta [Unconjugated]](https://images.novusbio.com/images2/IFNbeta_8499IF_1038.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")