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Recombinant Human MSS4 GST (N-Term) Protein

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12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, AP

Order Details

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Recombinant Human MSS4 GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-549 of Human PIP5K1B

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSSAAENGEAAPGKQNEEKTYKKTQVKNQILSRLPKIPCTFIQAWTNTEMITLLACYYFRSVSTASSAIKGAIQLGIGYTVGNLTSKPERDVLMQDFYVVESVFLPSEGSNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELSNPGASGSLFFVTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYKRRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLFTGIIDILQSYRLMKKLEHSWKALVYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSIAALKATSQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTPSLFEAASLATTISSSSLYVNEHYPHDRPTLYSNRFKMATSEH

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
PIP5K1B
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
88.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human MSS4 GST (N-Term) Protein

  • EC 2.7.1
  • MSS4Protein STM-7
  • Phosphatidylinositol-4-phosphate 5-kinase type I beta
  • phosphatidylinositol-4-phosphate 5-kinase type-1 beta
  • phosphatidylinositol-4-phosphate 5-kinase, type I, beta
  • PIP5K1-beta
  • PIP5KIbeta
  • PtdIns(4)P-5-kinase 1 beta
  • STM7EC 2.7.1.68
  • Type I phosphatidylinositol-4-phosphate 5-kinase beta

Background

MSS4, also known by its gene name PIP5K1B, has a 543 amino acid long isoform that is 61 kDa and a 549 amino acid isoform that is approximately 62 kDa. MSS4 plays a role in platelet adhesion, actin filament organization and the biosynthesis of PIP2. Current research on MSS4 is being conducted in relation to several diseases and disorders including kidney disease, schizophrenia, neuronitis and Friedreich's ataxia. MSS4 has also been shown to have interactions with CLIC1, BTK, MCRS1 and PIP5KL1 in pathways such as the RAC1 signaling pathway, CD28 signaling, Phosphatidylinositol signaling and the metabolism of lipids and lipoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PIP5K1B