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MSH6 Antibody (035)

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Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (035) [NBP2-89265] - Staining of MSH6 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated ...read more
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in Ferret kidney with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in human rectal cancer with rabbit monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in human colon carcinoma with rabbit monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in cynomolgus spleen with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in cynomolgus kidney with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in mouse spleen with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in mouse kidney with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in rat spleen with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in rat kidney with rabbit monoclonal antibody at 1:200 dilution
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in Ferret spleen with rabbit monoclonal antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma, PmSpecies Glossary
Applications ICC/IF, IHC
Clone
035
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MSH6 Antibody (035) Summary

Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human MSH6.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MSH6
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500

Reactivity Notes

Cross reactivity in IHC:
Cynomolgus, Mouse, Rat, Ferret

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for MSH6 Antibody (035)

  • DNA mismatch repair protein Msh6
  • G/T mismatch-binding protein
  • GTBPHNPCC5
  • GTMBP
  • hMSH6
  • HSAP
  • mutS (E. coli) homolog 6
  • mutS homolog 6 (E. coli)
  • MutS-alpha 160 kDa subunit
  • p160
  • sperm-associated protein

Background

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MSH6 Antibody (NBP2-89265) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for MSH6 Antibody (NBP2-89265). (Showing 1 - 1 of 1 FAQ).

  1. I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
    • Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

Secondary Antibodies

 

Isotype Controls

Additional MSH6 Products

Array NBP2-89265

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Bioinformatics

Gene Symbol MSH6