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MSH2 Antibody (rMSH2/6548)

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Western Blot: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Western blot analysis of HCT116 cell lysate using MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2/6548).

Product Details

Summary
Reactivity Hu, Ca, FeSpecies Glossary
Applications WB, IHC
Clone
rMSH2/6548
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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MSH2 Antibody (rMSH2/6548) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08956)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human MSH2 protein (Uniprot: IDP43246)
Localization
Nuclear
Marker
DNA Mismatch Repair Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSH2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MSH2 Antibody (rMSH2/6548)

  • COCA1
  • DNA mismatch repair protein Msh2
  • FCC1
  • hMSH2
  • HNPCC
  • HNPCC1
  • HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
  • LCFS2
  • MSH2
  • mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
  • MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MSH2