![Western Blot: Monkeypox Virus A4L Antibody (HL2555) - Azide and BSA Free [NBP3-25568] - Non-infected (-) and infected (+) BSC-40 whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Monkeypox virus A4L antibody [HL2555] (NBP3-25568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25568_rabbit-monkeypox-virus-a4l-mab-hl2555-azide-and-bsa-free-212024939356.jpg "Western Blot: Monkeypox Virus A4L Antibody (HL2555) - Azide and BSA Free [NBP3-25568] - Non-infected (-) and infected (+) BSC-40 whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Monkeypox virus A4L antibody [HL2555] (NBP3-25568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | HL2555 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Full length of Monkeypox Virus A4L recombinant protein (MPXV_USA_2022_MA001) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Monkeypox Virus A4L Antibody (HL2555) - Azide and BSA Free [NBP3-25568] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Monkeypox virus A4L antibody [HL2555] (NBP3-25568) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25568_rabbit-monkeypox-virus-a4l-mab-hl2555-azide-and-bsa-free-2120249413372.jpg "Western Blot: Monkeypox Virus A4L Antibody (HL2555) - Azide and BSA Free [NBP3-25568] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Monkeypox virus A4L antibody [HL2555] (NBP3-25568) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Monkeypox Virus A4L Antibody (HL2555) - Azide and BSA Free [NBP3-25568] - Monkeypox virus A4L antibody [HL2555] detects Monkeypox virus A4L protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: Monkeypox virus A4L stained by Monkeypox virus A4L antibody [HL2555] (NBP3-25568) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25568_rabbit-monkeypox-virus-a4l-mab-hl2555-azide-and-bsa-free-2120249351318.jpg "Immunocytochemistry/Immunofluorescence: Monkeypox Virus A4L Antibody (HL2555) - Azide and BSA Free [NBP3-25568] - Monkeypox virus A4L antibody [HL2555] detects Monkeypox virus A4L protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: Monkeypox virus A4L stained by Monkeypox virus A4L antibody [HL2555] (NBP3-25568) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")