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MLH3 Antibody

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Immunocytochemistry/ Immunofluorescence: MLH3 Antibody [NBP2-78702] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Flow Cytometry: MLH3 Antibody [NBP2-78702] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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MLH3 Antibody Summary

Immunogen
Peptide with sequence RQSLQQSMPPCEPP corresponding to C-Terminus according to NP_001035197.1, NP_055196.2.
Specificity
This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MLH3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MLH3 Antibody

  • DNA mismatch repair protein Mlh3
  • HNPCC7
  • MGC138372
  • mutL (E. coli) homolog 3
  • mutL homolog 3 (E. coli)
  • MutL protein homolog 3

Background

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for MLH3 Antibody (NBP2-78702) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MLH3