![Western Blot: MERS-CoV Nucleoprotein Antibody [NBP3-13422] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (NBP3-13422) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MERS-CoV-Nucleoprotein-Antibody-Western-Blot-NBP3-13422-img0002.jpg "Western Blot: MERS-CoV Nucleoprotein Antibody [NBP3-13422] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (NBP3-13422) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | VSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of MERS-CoV Nucleoprotein (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | N |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | N |
![Western Blot: MERS-CoV Nucleoprotein Antibody [NBP3-13422] - Non-infected and infected Vero B4 whole cell extracts (20 ug) were separated by gradient gel, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (NBP3-13422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MERS-CoV-Nucleoprotein-Antibody-Western-Blot-NBP3-13422-img0001.jpg "Western Blot: MERS-CoV Nucleoprotein Antibody [NBP3-13422] - Non-infected and infected Vero B4 whole cell extracts (20 ug) were separated by gradient gel, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (NBP3-13422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![ELISA: MERS-CoV Nucleoprotein Antibody [NBP3-13422] - Indirect ELISA analysis performed by coating plate with recombinant MERS-CoV Nucleocapsid protein (88.75-1.39 nM). Coated protein was probed with MERS-CoV Nucleoprotein antibody (NBP3-13422) (1 ug/mL). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.](http://images.novusbio.com/fullsize/MERS-CoV-Nucleoprotein-Antibody-ELISA-NBP3-13422-img0003.jpg "ELISA: MERS-CoV Nucleoprotein Antibody [NBP3-13422] - Indirect ELISA analysis performed by coating plate with recombinant MERS-CoV Nucleocapsid protein (88.75-1.39 nM). Coated protein was probed with MERS-CoV Nucleoprotein antibody (NBP3-13422) (1 ug/mL). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")