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Human MBL ELISA Kit (Colorimetric)

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ELISA: Human MBL ELISA Kit (Colorimetric) [NBP2-60089] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human MBL ELISA Kit (Colorimetric) [NBP2-60089] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human MBL ELISA Kit (Colorimetric) [NBP2-60089] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
1.563 ng/mL - 100 ng/mL
Sensitivity
0.171 ng/mL

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Human MBL ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human MBP/MBL. No significant cross-reactivity or interference between human MBP/MBL and analogues was observed.
Standard Curve Range
1.563 ng/mL - 100 ng/mL
Sensitivity
0.171 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MBL2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human MBL ELISA Kit (Colorimetric)

  • COLEC1
  • COLEC1collectin-1
  • Collectin-1
  • HSMBPC
  • Mannan-binding protein
  • mannose-binding lectin (protein C) 2, soluble (opsonic defect)
  • mannose-binding lectin (protein C) 2, soluble
  • Mannose-binding lectin
  • mannose-binding protein C
  • MBL
  • MBL2
  • MBL2D
  • MBLmannan-binding lectin
  • MBP
  • MBP1
  • MBP1mannose-binding lectin 2, soluble (opsonic defect)
  • MBP-C
  • MGC116832
  • MGC116833

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBP/MBL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP/MBL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP/MBL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP/MBL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MBL2