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Sheep Luteinizing Hormone ELISA Kit (Colorimetric)

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ELISA: Sheep Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60085] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Sheep Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60085] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sheep Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60085] - Standard Curve Reference

Product Details

Summary
Reactivity ShSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
1 mIU/mL - 50 mIU/mL
Sensitivity
0.5 mIU/mL

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Sheep Luteinizing Hormone ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of sheep LH. No significant cross-reactivity or interference between sheep LH and analogues was observed.
Standard Curve Range
1 mIU/mL - 50 mIU/mL
Sensitivity
0.5 mIU/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LHB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate
  4. Instruction manual
  5. Standard
  6. Stop Solution
  7. Substrate A
  8. Substrate B
  9. Wash Buffer (20 x concentrate)

Alternate Names for Sheep Luteinizing Hormone ELISA Kit (Colorimetric)

  • CGB4;HH23;LHB;LSH-B;LSH-beta;Lutropin subunit beta

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for LH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LHB