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Luciferase Antibody (965808) [Unconjugated]

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Western blot shows Recombinant Large BiT and Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB10026) followed by ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB
Clone
965808
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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Luciferase Antibody (965808) [Unconjugated] Summary

Immunogen
Synthetic peptide of NanoLuc® (Nluc) Luciferase
Specificity
Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. Detects NanoLuc® (Nluc) Luciferase and Large BiT in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB10026 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

NanoLuc and NanoBiT are registered trademarks of Promega Corporation.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Luciferase Antibody (965808) [Unconjugated]

  • ec 1.13.12.7
  • firefly luciferase
  • Fluc
  • LuC
  • Luciferase
  • luciferin 4 monooxygenase
  • Luciferin 4-monooxygenase

Background

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Luciferase Antibody (MAB10026)(4)

We have publications tested in 3 confirmed species: Human, Canine, Zebrafish.

We have publications tested in 1 application: Western Blot.


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Western Blot
(3)
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Human
(1)
Canine
(1)
Zebrafish
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Luciferase Antibody (MAB10026) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Luciferase Products

Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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