Luciferase Antibodies
Luciferase is a generic term for a group of oxidative enzymes that generate bioluminescence. Firefly and bacterial are two commonly used luciferase proteins. Firefly luciferase protein has a theoretical molecular weight of 61 kDa. We offer several antibodies to detect Luciferase that have been cited in 35+ publications with multiple 5-star reviews. Browse our catalog for recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and Luciferase-conjugated antibodies. Our Luciferase antibodies have been validated for multiple research applications including Dot Blot, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, and Western Blot. These Luciferase antibodies are suitable for use in Bacteria, Firefly, and Multi Species. Each Luciferase antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it. Choose the anti-Luciferase antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for Luciferase Antibodies
anti-Luciferase antibody, anti-ec 1.13.12.7 antibody, anti-firefly luciferase antibody, anti-Fluc antibody, anti-luciferin 4 monooxygenase antibody
10 Results for "luciferase" in Primary Antibodies
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0006.jpg "Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.")
![Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti-Luciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, 40X. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Immunohistochemistry-NB600-307-img0004.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573)](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0005.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416171421.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Induction of autophagy by TTFields is AMPK dependent.a U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of GFP78 protein. Numeric values represent fold increase in GRP78 signal, normalized to loading control (GAPDH), relative to untreated control. b Quantification of intracellular ATP levels in U-87 MG cells either left untreated or treated w/ TTFields for 72 h.Results presented as average ATP concentration (nmol/2 × 106 cells) from 3 independent experiments (*P < 0.01, Student’s t-test). c U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of pAMPK & pULK1proteins. GAPDH used as loading control. (5D-F) U-87 MG cells transfected w/ AMPK-targeting siRNA (siAMPK) or w/ siRNA sham vector (siVector), & incubated for 48 h w/ or w/out TTFields application. CQ 20 µM added for last 4 h of treatment where indicated. d (left panel) Immunoblot analysis of LC3 & AMPK. Numeric values represent fold-change in LC3-II signal, normalized to GAPDH signal, relative to respective control. d (right panel) CQ-treated cells fixed & stained for LC3 (green) & DAPI (blue), original magnifications: × 40. e Cell count of siAMPK- or siVector-expressing cells after TTFields treatment. (0.01 < *P < 0.05, Student’s t-test, n = 3). f siVector- & siAMPK-transfected U-87 MG cells either left untreated or treated w/ TTFields for 48 h. Cells then fixed & stained for cleaved caspase-3 (green) & DAPI (blue) (left panel). Images from each treatment analyzed manually & fraction of cleaved caspase-3-positive cells calculated for at least 200 cells from each group (right panel) (**P < 0.01, Student’s t-test, n = 2) Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616016.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163711.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Immunofluorescence imaging of luciferase protein as a reporter of hybrid formation. Entire primary tumor & lung sections were imaged via tile scanning, & each image of the scan was carefully analyzed to confirm or refute positive staining for luciferase. The luciferase signal was considered a positive signal if it was above background levels associated with negative controls & corresponded to the cytoplasm of a cell with a nucleus. Rare luciferase-positive cells were detected in the primary tumors. Most red signal was not in the cytoplasm of cells associated with nuclei and, therefore, considered nonspecific [insets (a), (c)]. The lungs containing metastases on the other hand [(b), (d)] contained a large number of bona fide luciferase-positive cells corresponding to fusion products. Scale bars on slide scans = 100 μm. Scale bars on 40× inset = 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31069316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241618921.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163757.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616033.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416175224.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NBP2-19295] - Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Firefly Luciferase stained by Firefly Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-19295_rabbit-polyclonal-luciferase-antibody-immunocytochemistry-immunofluorescence-298202414621..jpg "Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NBP2-19295] - Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Firefly Luciferase stained by Firefly Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.")
![Western Blot: Luciferase Antibody [NBP2-19295] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19295_rabbit-polyclonal-luciferase-antibody-western-blot-298202414820..jpg)
![Western Blot: Luciferase Antibody [NBP2-19295] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Luciferase antibody (NBP2-19295) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19295_rabbit-polyclonal-luciferase-antibody-9102024205670.jpg)
![Dot Blot: Luciferase Antibody [NBP2-44165] - Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Rabbit anti-Luciferase. Secondary Antibody: Goat Anti-Rabbit IgG HRP.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Dot-Blot-NBP2-44165-img0001.jpg "Dot Blot: Luciferase Antibody [NBP2-44165] - Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Rabbit anti-Luciferase. Secondary Antibody: Goat Anti-Rabbit IgG HRP.")
![Immunocytochemistry/Immunofluorescence: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Firefly Luciferase antibody [HL2164] detects Firefly Luciferase protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Firefly Luciferase stained by Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25468_rabbit-luciferase-mab-hl2164-azide-and-bsa-free-2120249403896.jpg "Immunocytochemistry/Immunofluorescence: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Firefly Luciferase antibody [HL2164] detects Firefly Luciferase protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Firefly Luciferase stained by Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Luciferase Antibody (HL2164) - Azide and BSA Free [NBP3-25468] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody [HL2164] (NBP3-25468) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25468_rabbit-luciferase-mab-hl2164-azide-and-bsa-free-2120249374961.jpg)
![Western Blot: Luciferase Antibody [NB110-17348] - Lysates of HEK-293T cells overexpressing luciferase was separated on SDS-PAGE and probed with Anti-Luciferase The antibody was developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:1,000 + luciferase immunizing peptide](http://images.novusbio.com/fullsize/Luciferase-Antibody-Western-Blot-NB110-17348-img0003.jpg "Western Blot: Luciferase Antibody [NB110-17348] - Lysates of HEK-293T cells overexpressing luciferase was separated on SDS-PAGE and probed with Anti-Luciferase The antibody was developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:1,000 + luciferase immunizing peptide")
![Immunocytochemistry/Immunofluorescence: Luciferase Antibody [NB110-17348] - HEK-293T cells overexpressing luciferase were fixed and stained 10 ug/mL Anti-Luciferase. The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-17348-img0004.jpg)
![Western Blot: Luciferase Antibody (Luci17) [NBP3-30808] - Western blot analysis using Luciferase antibody (Cat. No. NBP3-30808) on recombinant luciferase protein.](http://images.novusbio.com/fullsize/nbp3-30808_mouse-luciferase-mab-luci17-811202420592195.jpg "Western Blot: Luciferase Antibody (Luci17) [NBP3-30808] - Western blot analysis using Luciferase antibody (Cat. No. NBP3-30808) on recombinant luciferase protein.")