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Recombinant Human LMW-PTP/ACP1 His Protein

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SDS-Page: Recombinant Human LMW-PTP/ACP1 Protein [NBC1-21279] - Human, His-tagged, Recombinant, E.coli
SDS-Page: Recombinant Human LMW-PTP/ACP1 Protein [NBC1-21279] - Human, His-tagged, Recombinant, E.coli

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

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Recombinant Human LMW-PTP/ACP1 His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-158 of Human LMW-PTP/ACP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
Details of Functionality
Specific activity is > 15,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ACP1
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM MES (pH 6.0), 0.1 mM PMSF, 2 mM EDTA, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human LMW-PTP/ACP1 His Protein

  • acid phosphatase 1, soluble
  • acid phosphatase of erythrocyte
  • ACP1
  • Adipocyte acid phosphatase
  • cytoplasmic phosphotyrosyl protein phosphatase
  • EC 3.1.3.2
  • EC 3.1.3.48
  • HAAP
  • LMWPTP
  • LMW-PTP
  • LMW-PTPase
  • Low molecular weight cytosolic acid phosphatase
  • low molecular weight phosphotyrosine protein phosphatase
  • MGC111030
  • MGC3499
  • protein tyrosine phosphatase
  • Red cell acid phosphatase 1

Background

Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ACP1