![Western Blot: Influenza A M1 Antibody (1G1A12) - BSA Free [NBP2-50480] - Analysis of Influenza A matrix protein in Cos-7 cells transfected or mock-transfected with a plasmid containing the M1 open reading frame.](http://images.novusbio.com/fullsize/Influenza-A-M1-Antibody-1G1A12-BSA-Free-Western-Blot-NBP2-50480-img0008.jpg "Western Blot: Influenza A M1 Antibody (1G1A12) - BSA Free [NBP2-50480] - Analysis of Influenza A matrix protein in Cos-7 cells transfected or mock-transfected with a plasmid containing the M1 open reading frame.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, Func, ICC/IF |
| Clone | 1G1A12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | GST-Influenza A M1 fusion protein |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Positive control(s): Transfected COS-7 cells. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Influenza A M1 Antibody (1G1A12) - BSA Free [NBP2-50480] - Immunofluorescence on cos-7 cells transfected with a plasmid containing the M1 open reading frame using anti-M1 [1G1A12], Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and stained with anti-M1 [1G1A12] followed by FITC-conjugated secondary goat anti-mouse. DAPI counterstain is shown in blue (left panel) and antibody staining in green (middle panel).](http://images.novusbio.com/fullsize/Influenza-A-M1-Antibody-1G1A12-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-50480-img0007.jpg "Immunocytochemistry/Immunofluorescence: Influenza A M1 Antibody (1G1A12) - BSA Free [NBP2-50480] - Immunofluorescence on cos-7 cells transfected with a plasmid containing the M1 open reading frame using anti-M1 [1G1A12], Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and stained with anti-M1 [1G1A12] followed by FITC-conjugated secondary goat anti-mouse. DAPI counterstain is shown in blue (left panel) and antibody staining in green (middle panel).")
![Western Blot: Influenza A M1 Antibody (1G1A12) - BSA Free [NBP2-50480] - Western blotting (A) or ELISA (B) on a lysate prepared from MDCK cells either mock infected or infected with different strains of influenza A virus.](http://images.novusbio.com/fullsize/Influenza-A-M1-Antibody-1G1A12-BSA-Free-Western-Blot-NBP2-50480-img0004.jpg "Western Blot: Influenza A M1 Antibody (1G1A12) - BSA Free [NBP2-50480] - Western blotting (A) or ELISA (B) on a lysate prepared from MDCK cells either mock infected or infected with different strains of influenza A virus.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")