ILT1/CD85h/LILRA2 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

LILRA2, also known as Leukocyte immunoglobulin-like receptor subfamily A member 2, has a 483 amino acid long isoform that is 53 kDa and a short 466 amino acid isoform that is 51 kDa, acts as an activating receptor for eosinophils that elicited the release of cytotoxic granule proteins, de novo lipid mediator generation, and cytokine release through vesicular transport, but does not bind class I MHC antigens. Studies are being performed in relation to this protein and several diseases and disorders including leprosy, microscopic polyangiitis, systemic lupus, erythematosus, lupus erythematosus, hepatitis c, and hepatitis b. This protein has been shown to interact with EWSR1 and USP46 proteins in osteoclast differentiation, defense response, and signal transduction pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-07570

• ILT1/CD85h/LILRA2 Antibodies
• ILT1/CD85h/LILRA2 Lysate
• ILT1/CD85h/LILRA2 Proteins and Enzyme