IL-32 Antibody (403) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ELISA
Clone
403
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 646

Order Details

IL-32 Antibody (403) [Janelia Fluor® 646] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human IL-32 (Accession#: NP_001012651.1; Met1-Lys131).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IL32
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA Detection
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IL-32 Antibody (403) [Janelia Fluor® 646]

  • IL32
  • IL-32
  • IL-32alpha
  • IL-32beta
  • interleukin 32
  • interleukin-32 theta
  • interleukin-32
  • natural killer cell transcript 4
  • Natural killer cells protein 4
  • NK4
  • NK4IL-32delta
  • TAIF
  • TAIFa
  • TAIFb
  • TAIFc
  • TAIFd
  • TAIFIL-32gamma
  • Tumor necrosis factor alpha-inducing factor

Background

IL32 alfa is the shortest of the 4 isotypes of IL32 known, it is 134 amino acids ( kDa) protein, the IL32B (beta) is 188, IL32C is 168 and IL32D is 179 amino acids long proteins. Each subtype has an N-terminal segment and four kringle domains (NK4), that interact with several other proteins including the hepatic growth factor via c-met a tyrosine receptor kinase (5). IL-32 synergized with the intracellular nuclear oligomerization domain receptors (NOD1- and NOD2-specific muropeptides of peptidoglycans for the release of IL-1beta and IL-6. In contrast, IL-32 did not influence the cytokine production induced via TLRs (6). The anti-IL32 selective antibodies were made against an epitope that lies near the C-terminal end of the protein. The antibodies to IL32 are affinity purified on immobilized affinity based chromatography and characterized for applications in ELISA and Western blotting. The antiIL32 antibodies recognize a single band of IL32 in PC-IL32 samples. The IL32 antibodies do not cross react with other pro-inflammatory or anti-inflammatory interleukins, has also produced antibodies to other interleukins, interleukin receptors, cytokines and their receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL32