Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IGF-II R/IGF2R Antibody (372616) [Unconjugated]
300 kDa mannose 6-phosphate receptor
cation-independent mannose-6 phosphate receptor
cation-independent mannose-6-phosphate receptor
CD222 antigen
CD222
CI Man-6-P receptor
CIMPR
CI-MPR
IGF2R
IGF-II R
IGF-II receptor
IGFIIR
IGF-IIR
insulin-like growth factor 2 receptorM6P/IGF2R
Insulin-like growth factor II receptor
M6P/IGF2 receptor
M6PR
M6P-R
MPR 300
MPR1
MPRI
MPRIM6PR
Background
The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1-3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous "binding" repeats of about 150 aa each. The odd-numbered repeats interact with "ligands" while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P-containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G‑proteins, an effect that has yet to be conclusively shown (6).
Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
Morgan, D.O. et al. (1987) Nature 329:301.
Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IGF-II R/IGF2R Antibody (MAB24471)(1)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 1 application: ELISA Development (Capture).
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our IGF-II R/IGF2R Antibody (372616) [Unconjugated] and receive a gift card or discount.
