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Iduronate 2-Sulfatase/IDS Antibody

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Western Blot: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477] - (3.8ug/ml) staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Iduronate 2-Sulfatase/IDS Antibody Summary

Immunogen
Peptide with sequence C-KHFRFRDLEEDP corresponding to internal region according to NP_000193.1.
Specificity
This antibody is expected to recognize isoform a (NP_000193.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IDS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human liver lysates (calculated MW of 61.9 kDa band according to NP_000193.1). IHC-P: Human placenta shows lysosomal staining of trophoblasts.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-28477.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody

  • Alpha-L-iduronate sulfate sulfatase
  • EC 3.1.6.13
  • IDS
  • iduronate 2-sulfatase 14 kDa chain
  • iduronate 2-sulfatase 42 kDa chain
  • iduronate 2-sulfatase
  • idursulfase
  • MPS2
  • S
  • SIDS

Background

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Iduronate 2-Sulfatase/IDS Antibody (NBP1-28477)(1)

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Bioinformatics

Gene Symbol IDS
Entrez
Uniprot