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Human NT-ProANP Quantikine ELISA Kit

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JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human NT-ProANP Quantikine ELISA Kit Summary

Background
The Quantikine® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the sta...ndard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.
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Specificity
Natural and recombinant human NT-ProANP.
Details of Functionality
None<br /><br />
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DANP00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human NT-ProANP Quantikine ELISA Kit

  • ANF
  • ANP
  • ANPatriopeptin
  • ATFB6
  • atrial natriuretic factor
  • Atrial Natriuretic Peptide
  • Cardionatrin
  • CDD-ANF
  • natriuretic peptide A
  • natriuretic peptide precursor A
  • NPPA
  • PND
  • PNDcardionatrin
  • prepronatriodilatin

Background

Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.

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&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Atrial Natriuretic Peptide/ANP (DANP00)(3)

We have publications tested in 1 confirmed species: Human.


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